sod(VI)C is an alpha-COP-related gene which is essential for establishing and maintaining polarized growth in Aspergillus nidulans

Citation
Sl. Whittaker et al., sod(VI)C is an alpha-COP-related gene which is essential for establishing and maintaining polarized growth in Aspergillus nidulans, FUNGAL G B, 26(3), 1999, pp. 236-252
Citations number
61
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences",Microbiology
Journal title
FUNGAL GENETICS AND BIOLOGY
ISSN journal
1087-1845 → ACNP
Volume
26
Issue
3
Year of publication
1999
Pages
236 - 252
Database
ISI
SICI code
1087-1845(199904)26:3<236:SIAAGW>2.0.ZU;2-Z
Abstract
Strains of Aspergillus nidulans carrying the conditional-lethal mutation so d(VI)C1 (stabilization of disomy) are defective in nuclear division and hyp hal extension. The mutation affects both the establishment and maintenance of polar growth, since mutant spores do not germinate at restrictive temper ature and preexisting hyphae stop growing upon upshift, The defect is rever sible within the first 3-4 h at restrictive temperature but longer periods of incubation are lethal due to cell lysis and morphological abnormalities, There is no evidence for a specific cell cycle lesion, suggesting the exis tence of a feedback mechanism whereby hyphal extension is coordinated with nuclear partitioning. The sod(VI)C gene has been cloned from a chromosome V I-specific cosmid library and its product exhibits strong homology to the a lpha-COP subunit of the coatomer complex involved in the secretory pathway in yeast and higher organisms. Molecular disruption of the gene is lethal, indicating that Sod(VI)C is essential for growth in A. nidulans, (C) 1999 A cademic Press.