Methylprednisolone and vitamin E therapy in perinatal hypoxic-ischemic brain damage in rats

Citation
M. Daneyemez et al., Methylprednisolone and vitamin E therapy in perinatal hypoxic-ischemic brain damage in rats, NEUROSCIENC, 92(2), 1999, pp. 693-697
Citations number
46
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROSCIENCE
ISSN journal
0306-4522 → ACNP
Volume
92
Issue
2
Year of publication
1999
Pages
693 - 697
Database
ISI
SICI code
0306-4522(1999)92:2<693:MAVETI>2.0.ZU;2-7
Abstract
To study the efficacy of methylprednisolone/vitamin E in reducing cerebral edema and improving the ultimate neuropathological outcome in perinatal cer ebral hypoxia-ischemia, 40 seven-day postnatal rats were subjected to right common carotid artery ligation followed by exposure to 8% oxygen at 37 deg rees C for 3 h. The animals were divided into groups. Twenty rat pups recei ved an intraperitoneal injection of 30 mg/kg body weight methylprednisolone and vitamin E (100 U/kg) immediately following cerebral hypoxia-ischemia. Control animals received either no therapy (n = 10) or an equivalent volume of normal saline (n = 10). After 72 h of recovery from hypoxia-ischemia, t he animals were killed and their brains were examined to measure the water contents in the right and left hemispheres (29 rat pups), whereas the other s were killed at 21 days for neuropathological examination. Methylprednisol one/vitamin E-treated rats had significantly less water content in the righ t hemisphere (87.08 +/- 0.28%, mean +/- S.E.M.) than saline-treated animals (89.07 +/- 0.37%, mean +/- S.E.M., P < 0.0001). Methylprednisolone/vitamin E significantly reduced water content in the right hemisphere of the brain . Neuropathological study was performed on nine rat pups. The brains of fou r methylprednisolone/vitamin E- and five saline-treated pups were examined at the end of the 21-day recovery period. Two groups of the right cerebral cortex included thinning of the cortex. Significantly less damage was seen in the methylprednisolone/vitamin E-treated pups. Our study suggests that trials of methylprednisolone/vitamin E might be eff ective if they are given to the mother at risk of fetal hypoxia during labo r or to the hypoxic infant right after delivery in preventing hypoxic brain damage. (C) 1999 IBRO. Published by Elsevier Science Ltd.