The intrinsic factor-vitamin B-12 receptor, cubilin, is a high-affinity apolipoprotein A-I receptor facilitating endocytosis of high-density lipoprotein

Citation
R. Kozyraki et al., The intrinsic factor-vitamin B-12 receptor, cubilin, is a high-affinity apolipoprotein A-I receptor facilitating endocytosis of high-density lipoprotein, NAT MED, 5(6), 1999, pp. 656-661
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research General Topics
Journal title
NATURE MEDICINE
ISSN journal
1078-8956 → ACNP
Volume
5
Issue
6
Year of publication
1999
Pages
656 - 661
Database
ISI
SICI code
1078-8956(199906)5:6<656:TIFBRC>2.0.ZU;2-O
Abstract
Cubilin is the intestinal receptor for the endocytosis of intrinsic factor- vitamin B-12. However, several lines of evidence, including a high expressi on in kidney and yolk sac, indicate it may have additional functions. We is olated apolipoprotein A-I (apoA-I), the main protein of high-density lipopr otein (HDL), using cubilin affinity chromatography. Surface plasmon resonan ce analysis demonstrated a high-affinity binding of apoA-I and HDL to cubil in, and cubilin-expressing yolk sac cells showed efficient I-125-HDL endocy tosis that could be inhibited by IgG antibodies against apoA-I and cubilin. The physiological relevance of the cubilin-apoA-I interaction was further emphasized by urinary apoA-I loss in some known cases of functional cubilin deficiency. Therefore, cubilin is a receptor in epithelial apoA-I/HDL meta bolism.