Membrane tubule-mediated reassembly and maintenance of the Golgi complex is disrupted by phospholipase A(2) antagonists

Citation
P. De Figueiredo et al., Membrane tubule-mediated reassembly and maintenance of the Golgi complex is disrupted by phospholipase A(2) antagonists, MOL BIOL CE, 10(6), 1999, pp. 1763-1782
Citations number
77
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
MOLECULAR BIOLOGY OF THE CELL
ISSN journal
1059-1524 → ACNP
Volume
10
Issue
6
Year of publication
1999
Pages
1763 - 1782
Database
ISI
SICI code
1059-1524(199906)10:6<1763:MTRAMO>2.0.ZU;2-C
Abstract
Although membrane tubules can be found extending from, and associated with, the Golgi complex of eukaryotic cells, their physiological function has re mained unclear. To gain insight into the biological significance of membran e tubules, we have developed methods for selectively preventing their forma tion. We show here that a broad range of phospholipase A, (PLA,) antagonist s not only arrest membrane tubule-mediated events that occur late in the as sembly of the Golgi complex but also perturb its normal steady-state tubulo vesicular architecture by inducing a reversible fragmentation into separate "mini-stacks." In addition, we show that these same compounds prevent the formation of membrane tubules from Golgi stacks in an in vitro reconstituti on system. This in vitro assay was further used to demonstrate that the rel evant PLA, activity originates from the cytoplasm. Taken together, these re sults demonstrate that Golgi membrane tubules, sensitive to potent and sele ctive PLA, antagonists, mediate both late events in the reassembly of the G olgi complex and the dynamic maintenance of its steady-state architecture. In addition, they implicate a role for cytoplasmic PLA, enzymes in mediatin g these membrane trafficking events.