Sedimentation field-flow fractionation application to Toxoplasma gondii separation and purification

Citation
F. Bouamrane et al., Sedimentation field-flow fractionation application to Toxoplasma gondii separation and purification, J PHARM B, 20(3), 1999, pp. 503-512
Citations number
35
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
ISSN journal
0731-7085 → ACNP
Volume
20
Issue
3
Year of publication
1999
Pages
503 - 512
Database
ISI
SICI code
0731-7085(199907)20:3<503:SFFATT>2.0.ZU;2-M
Abstract
Toxoplasmosis is a worldwide disease caused by Toxoplasma gondii, an intrac ellular protozoa of micronic size range (4-10 mu m). Its classical purifica tion processes are complex and often associated with low recovery. All inve stigation procedures concerning this parasite require its isolation and pur ification from at least the mouse ascitic fluid. For this purpose, a recent ly developed laboratory technology was used, i.e. sedimentation field-flow fractionation. This chromatographic-like separation technology was demonstr ated to be particularly selective for isolation and separation of micron-si zed biological particles. Sedimentation field-flow fractionation operated o n the steric-hyper-layer mode was used to isolate the parasite from the rem anent ascitic contaminants of different origins and from red blood cells. W ith this technology, 86% recovery with 97% viability was obtained in less t han 30 min. (C) 1999 Elsevier Science B.V. All rights reserved.