Molecular and epidemiological characterization of vaginal Saccharomyces cerevisiae isolates

Citation
B. Posteraro et al., Molecular and epidemiological characterization of vaginal Saccharomyces cerevisiae isolates, J CLIN MICR, 37(7), 1999, pp. 2230-2235
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
0095-1137 → ACNP
Volume
37
Issue
7
Year of publication
1999
Pages
2230 - 2235
Database
ISI
SICI code
0095-1137(199907)37:7<2230:MAECOV>2.0.ZU;2-0
Abstract
Although vaginitis caused by Saccharomyces cerevisiae is extremely rare, in recent years we have experienced an increasing frequency of S. cerevisiae isolation from the vaginas of fertile-age women. In order to investigate th e epidemiology of these vaginal infections, a total of 40 isolates of S. ce revisiae derived from symptomatic and asymptomatic women were characterized by two DNA typing approaches, named ribosomal DNA (rDNA) hybridization and Ty917 hybridization, based on the Southern blotting technique. After trans fer, the polymorphic DNA restriction fragments were hybridized with the ent ire repeat of S. cerevisiae rDNA for one method and with the entire sequenc e of the Ty917 retrotransposon for the other. after elaboration with comput er assisted analysis, the results of each method showed that Ty917 hybridiz ation is endowed with a discriminatory power higher than that of rDNA hybri dization, With the Ty917 hybridization method, all of the S. cerevisiae iso lates tested appeared very heterogeneous, with the exception of those colle cted from individual patients with recurrent vaginitis. This allowed us to exclude a possible common source of infection while the high relatedness am ong S, cerevisiae sequential isolates front recurrent-vaginitis patients co uld suggest a pattern of relapse rather than frequent reinfection.