Two genes control aluminum tolerance in maize: Genetic and molecular mapping analyses

Citation
St. Sibov et al., Two genes control aluminum tolerance in maize: Genetic and molecular mapping analyses, GENOME, 42(3), 1999, pp. 475-482
Citations number
61
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,"Molecular Biology & Genetics
Journal title
GENOME
ISSN journal
0831-2796 → ACNP
Volume
42
Issue
3
Year of publication
1999
Pages
475 - 482
Database
ISI
SICI code
0831-2796(199906)42:3<475:TGCATI>2.0.ZU;2-S
Abstract
We have identified two loci linked to aluminum (Al) tolerance in the maize inbred line Cat-100-6 by means of restriction fragment length polymorphism (RFLP) and bulked segregant analysis (BSA). A segregating population F-2 wa s obtained from a cross between Cat-100-6 (Al tolerant) x S1587-17 (Al sens itive) parents. Subsequently two DNA bulks of individuals, displaying a con trasting Al tolerance trait were generated from Fz. From a total of 158 mar kers used, 30 markers were identified showing polymorphism between parents and bulks. The segregation results derived from the hybridization from thes e 30 markers and 56 individuals from F-2 revealed 10 markers cosegregating with the Al tolerance which were located in two linkage groups. The linkage groups were composed of 6 and 4 markers, and they were mapped on the short arm of chromosomes 6 and 10, respectively. From these observations, we ded uce that two loci are involved in this trait in Cat-100-6 line. QGENE softw are was used to study the correlation between these two loci and the trait for aluminum tolerance. The results indicate that the locus on chromosome 1 0 has the stronger effect, and it is responsible for the major part of the variability of the trait.