Independent evolution of HIV type 1 in different brain regions

Citation
P. Shapshak et al., Independent evolution of HIV type 1 in different brain regions, AIDS RES H, 15(9), 1999, pp. 811-820
Citations number
71
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
AIDS RESEARCH AND HUMAN RETROVIRUSES
ISSN journal
0889-2229 → ACNP
Volume
15
Issue
9
Year of publication
1999
Pages
811 - 820
Database
ISI
SICI code
0889-2229(19990610)15:9<811:IEOHT1>2.0.ZU;2-0
Abstract
HIV-1-associated brain pathology exhibits regional variability and we there fore studied the genetic differences in the V1-V5 domains of the HIV env ge ne in up to four regions of brain (frontal lobe, basal ganglia, medial temp oral lobe, and nonmedial temporal lobe) from three patients. We found that in each separate brain region HIV-1 forms different quasispecies and that t here is little gene flow among these regions, In further support of brain r egion-specific evolution of HIV-1, we analyzed amino acid signatures in the se clones. In addition to known amino acid signatures associated with macro phage tropism and the lack of syncytium formation, we found 15 majority ami no acid signature patterns from the V1-V5 env sequences associated with the neuroanatomical regions analyzed from the three individuals. Furthermore, on average, intrabrain genetic distances for the HIV-1 env were estimated t o be much smaller than genetic distances between brain regions. Specific st rains of HIV-1 may be neurotropic or neuroinvasive (replication preference in brain tissue) and may contribute to pathology, cognitive loss, and neuro psychiatric disease.