Immunohistochemical study of sialyl Le(a) and sialyl Le(x) antigen in oralsquamous cell carcinoma: The association of sialyl Le(a) expression with the metastatic potential

Citation
S. Kurahara et al., Immunohistochemical study of sialyl Le(a) and sialyl Le(x) antigen in oralsquamous cell carcinoma: The association of sialyl Le(a) expression with the metastatic potential, HEAD NECK, 21(4), 1999, pp. 330-337
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Otolaryngology
Journal title
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
ISSN journal
1043-3074 → ACNP
Volume
21
Issue
4
Year of publication
1999
Pages
330 - 337
Database
ISI
SICI code
1043-3074(199907)21:4<330:ISOSLA>2.0.ZU;2-I
Abstract
Background. Carbohydrate antigens in cancer cells are considered to be invo lved in the binding of cancer cells to the endothelium during metastasis. Methods. Seventy cases of primary oral squamous cell carcinoma (SCC) were o btained from biopsy specimens and were analyzed immunohistochemically using an antibody against sialyl Lewis (Le)(a) or sialyl Le(x). Flow cytometry w as performed to detect the sialyl Le(a) or sialyl Le(x) expressed on oral S CC cell lines. Results. The expressions of sialyl Le(a), but not sialyl Le(x), of primary tumors significantly correlated to nodal metastasis; 71% of the metastatic cases express sialyl Le(a) and the cases with positive sialyl Le(a) and no sialyl Le(x) demonstrated a high incidence of metastasis (80%). A flow cyto metric study demonstrated the oral SCC cell line, which can metastasize in nude mice, to express a high level of sialyl Le(a). Conclusion. The high expression of sialyl Le(a) in primary tumors may thus be involved in nodal metastasis and therefore predict a poor prognosis in o ral SCC. (C) 1999 John Wiley & Sons, Inc.