Evaluation of a novel ELISA screening test for detection of factor VIII inhibitory antibodies in haemophiliacs

Citation
Pg. Martin et al., Evaluation of a novel ELISA screening test for detection of factor VIII inhibitory antibodies in haemophiliacs, CLIN LAB H, 21(2), 1999, pp. 125-128
Citations number
10
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Hematology
Journal title
CLINICAL AND LABORATORY HAEMATOLOGY
ISSN journal
0141-9854 → ACNP
Volume
21
Issue
2
Year of publication
1999
Pages
125 - 128
Database
ISI
SICI code
0141-9854(199904)21:2<125:EOANES>2.0.ZU;2-B
Abstract
Treatment of patients with haemophilia A with coagulation factor concentrat es may result in the development of inhibitory antibodies directed against factor VIII (FVIII). In this study, a previously unpublished ELISA test for FVIII inhibitor screening (Genetic Testing Institute [GTI] FVIII inhibitor , Brookfield, WI, USA) was evaluated in 131 blood samples (124 samples from patients with haemophilia A, and seven serial samples from one patient wit h an acquired FVIII inhibitor). Comparisons were made with the routine scre ening assay (based on recovery of FVIII) and confirmed where positive ( < 9 0% recovery) with the New Oxford assay, The ELISA kit had a sensitivity of 97.7% and specificity of 78.4%. The high negative predictive value of this new test (98.6%) suggests it may be useful as a reliable, rapid (< 2 h) and flexible (microwell strip format) tool for inhibitor screening of samples from both patients with haemophilia A and those with suspected acquired FVI II inhibitors.