Detection of chromosome 11q13 amplification in head and neck cancer using fluorescence in situ hybridization

Citation
Mb. Wang et al., Detection of chromosome 11q13 amplification in head and neck cancer using fluorescence in situ hybridization, ANTICANC R, 19(2A), 1999, pp. 925-931
Citations number
36
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
ANTICANCER RESEARCH
ISSN journal
0250-7005 → ACNP
Volume
19
Issue
2A
Year of publication
1999
Pages
925 - 931
Database
ISI
SICI code
0250-7005(199903/04)19:2A<925:DOC1AI>2.0.ZU;2-K
Abstract
Background: Head and neck squamous cell carcinoma (HNSCC) remains a cancer with one of the lowest five-year survival rates. Despite a better understan ding of the disease and recent advances in diagnosis and treatment, surviva l rates for HNSCC patients have not improved. Chromosomal abnormalities are common in HNSCC, and aberrations of chromosome 11q13 have been correlated with a poor prognosis. Materials and Methods: In this study we utilized flu orescence in situ hybridization (FISH) to determine the incidence of 11q13 amplification in twenty primary HNSCC tumors. INT-2 was used as the 11q13 p robe, and 9 and 11 centromeric probes were used as controls. Results: Polys omy, greater than two copies of chromosome 11, was found in 2 of 20 tumors. INT2 (11q13) amplification was found in 3 other tumors. Conclusions: These preliminary studies indicate that analysis of a larger sample of tumors us ing FISH may yield important diagnostic and prognostic information about he ad and neck tumors.