D. Grisaru et al., Placing the newborn on the maternal abdomen after delivery increases the volume and CD34(+) cell content in the umbilical cord blood collected: An old maneuver with new applications, AM J OBST G, 180(5), 1999, pp. 1240-1243
OBJECTIVE: Our purpose was to increase the number of the progenitor cells i
n umbilical cord blood collected for transplantation.
STUDY DESIGN: We randomly assessed the effect of "upper" and "lower" positi
ons of the newborn on the volume and progenitor cell (CD34(+)) content of t
he umbilical cord blood collected from 49 healthy, vaginally delivered, ter
RESULTS: Twenty-two collections were performed in the "upper" and 27 in the
"lower" position. The volume of umbilical cord blood obtained in the "uppe
r' position was 108.1 +/- 19.1 mL compared with 42.6 +/- 19.5 mt in the "lo
wer" position (P <.0001). Mononuclear cell separation revealed significantl
y higher numbers of cells in umbilical cord blood obtained in the "upper" g
roup (P<.01). Although the percentage of CD34+ cells was comparable, the ab
solute number of CD34+ cells was significantly higher in the "upper" group
because of the larger volume collected (P <.02). At 24 hours after delivery
the hemoglobin levels were not significantly different between newborns of
the 2 groups.
CONCLUSIONS: Placing the newborn on the maternal abdomen after delivery and
before cord clamping may significantly increase the volume of umbilical co
rd blood collected and therefore the CD34(+) counts that improve transplant
ation success without placing the mother or the newborn at risk. (Am J Obst
et Gynecol 1999;180:1240-3.).