Butane metabolism by butane-grown 'Pseudomonas butanovora'

Authors
Citation
Dj. Arp, Butane metabolism by butane-grown 'Pseudomonas butanovora', MICROBIO-UK, 145, 1999, pp. 1173-1180
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
MICROBIOLOGY-UK
ISSN journal
1350-0872 → ACNP
Volume
145
Year of publication
1999
Part
5
Pages
1173 - 1180
Database
ISI
SICI code
1350-0872(199905)145:<1173:BMBB'B>2.0.ZU;2-D
Abstract
The pathway of butane metabolism by butane-grown 'Pseudomonas butanovora' w as determined to be butane --> 1-butanol --> butyraldehyde --> butyrate. Bu tane was initially oxidized at the terminal carbon to produce 1-butanol. Up to 90% of the butane consumed was accounted for as 1-butanol when cells we re incubated in the presence of 5 mM 1-propanol (to block subsequent metabo lism of 1-butanol). No production of the subterminal oxidation product, 2-b utanol, was detected, even in the presence of 5 mM 2-pentanol (an effective inhibitor of 2-butanol consumption). Ethane, propane and pentane, but not methane, were also oxidized. Butane-grown cells consumed 1-butanol and othe r terminal alcohols. Secondary alcohols, including 2-butanol, were oxidized to the corresponding ketones. Butyraldehyde was further oxidized to butyra te as demonstrated by blocking butyrate metabolism with 1 mM sodium valerat e. Butyrate also accumulated from butane when cells were incubated with 1 m M sodium valerate. The pathway intermediates (butane, 1-butanol, butyraldeh yde and butyrate) and 2-butanol stimulated O-2 consumption by butane-grown cells, 1-Butanol, butyraldehyde and butyrate supported growth of 'P. butano vora', as did 2-butanol and lactate.