PCR-restriction enzyme analysis gor detection of Candida DNA in blood fromfebrile patients with hematological malignancies

Citation
G. Morace et al., PCR-restriction enzyme analysis gor detection of Candida DNA in blood fromfebrile patients with hematological malignancies, J CLIN MICR, 37(6), 1999, pp. 1871-1875
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
0095-1137 → ACNP
Volume
37
Issue
6
Year of publication
1999
Pages
1871 - 1875
Database
ISI
SICI code
0095-1137(199906)37:6<1871:PEAGDO>2.0.ZU;2-Y
Abstract
Blood samples were drawn daily from 72 patients who had hematological malig nancies, neutropenia, and fever and who had failed to respond to broad-spec trum antibiotics. Each sample was used for conventional fungal blood cultur es and for detection and identification of Candida DNA by a PCR method with subsequent restriction enzyme analysis (REA) recently developed in our lab oratory. The PCR method was able to detect five CFU of Candida spp. per mi of blood, and subsequent REA of the amplicons allowed the identification of the Candida species most commonly implicated in cases of candidiasis. Thir ty-one patients were PCR-REA positive, and four of these patients were also culture positive. The ultimate diagnosis for 13 of these patients and 1 pa tient who was PCR-REA negative was disseminated candidiasis (confirmed by c linical data, multiple cultures, histology, autopsy, and/or ultrasonographi c evidence of hepatosplenic candidiasis). The molecular method is significa ntly more sensitive than conventional fungal blood cultures and has a high negative predictive value (97.5%) for the development of disseminated candi diasis in neutropenic patients.