A radiation hybrid map of the rat genome containing 5,255 markers

Citation
Tk. Watanabe et al., A radiation hybrid map of the rat genome containing 5,255 markers, NAT GENET, 22(1), 1999, pp. 27-36
Citations number
44
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
NATURE GENETICS
ISSN journal
1061-4036 → ACNP
Volume
22
Issue
1
Year of publication
1999
Pages
27 - 36
Database
ISI
SICI code
1061-4036(199905)22:1<27:ARHMOT>2.0.ZU;2-O
Abstract
A whole-genome radiation hybrid (RH) panel was used to construct a high-res olution map of the rat genome based on microsatellite and gene markers. The se include 3,019 new microsatellite markers described here for the first ti me and 1,714 microsatellite markers with known genetic locations, allowing comparison and integration of maps from different sources. A robust RH fram ework map containing 1,030 positions ordered with odds of at least 1,000:1 has been defined as a tool for mapping these markers, and for future RH map ping in the rat, More than 500 genes which have been mapped in mouse and/or human were localized with respect to the rat RH framework, allowing the co nstruction of detailed rat-mouse and rat-human comparative maps and illustr ating the power of the RH approach for comparative mapping.