Serial lectin affinity chromatography demonstrates altered asparagine-linked sugar-chain structures of prostate-specific antigen in human prostate carcinoma

Citation
S. Sumi et al., Serial lectin affinity chromatography demonstrates altered asparagine-linked sugar-chain structures of prostate-specific antigen in human prostate carcinoma, J CHROMAT B, 727(1-2), 1999, pp. 9-14
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
JOURNAL OF CHROMATOGRAPHY B
ISSN journal
1387-2273 → ACNP
Volume
727
Issue
1-2
Year of publication
1999
Pages
9 - 14
Database
ISI
SICI code
1387-2273(19990430)727:1-2<9:SLACDA>2.0.ZU;2-N
Abstract
Differences between prostate carcinoma (PCA) and benign prostatic hyperplas ia (BPH) in asparagine (N)-linked sugar-chain structures of prostate-specif ic antigen (PSA) were investigated using serial lectin affinity chromatogra phy. The amounts of PSA passing through columns of concanavalin A (Con A), phytohaemagglutinin E4 (PHA-E4) and PHA-L4 were significantly greater for P CA-derived PSA than BPH. We propose that the sugar moiety structure of PSA which is increased in PCA is a multiantennary complex type with branched N- acetylglucosamine beta(1-->4) mannose. We suggest that N-linked sugar chain s in PSA are altered during oncogenesis in the human prostate and may serve as diagnostic tools for PCA. (C) 1999 Elsevier Science BN. All rights rese rved.