Detection of phosphatidylserine surface exposure on human erythrocytes using annexin V-ferrofluid

Citation
D. Geldwerth et al., Detection of phosphatidylserine surface exposure on human erythrocytes using annexin V-ferrofluid, BIOC BIOP R, 258(1), 1999, pp. 199-203
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006-291X → ACNP
Volume
258
Issue
1
Year of publication
1999
Pages
199 - 203
Database
ISI
SICI code
0006-291X(19990429)258:1<199:DOPSEO>2.0.ZU;2-3
Abstract
The asymmetric transbilayer distribution of phospholipids in the plasma mem brane and the regulation of phosphatidylserine (PS) exposure at the cell su rface of animal cells are of high physiological significance. It has been s hown previously that annexin V is one of the most sensitive tools with whic h the presence of small amounts of PS on the outer surface of eukaryotic ce lls can be detected. We present here the covalent coupling of annexin V mol ecules to magnetic nanoparticles of maghemite. The resulting annexin V-ferr ofluid is used in the magnetic separation of PS exposing cells, as illustra ted for human erythrocytes modified in their phospholipid transbilayer asym metry by the use of a calcium ionophore. Results on stored human erythrocyt es and comparison with results obtained using iodinated and fluorescein-lab eled annexin V are also presented. (C) 1999 Academic Press.