Detection of dexamethasone in the cornea and lens by NMR spectroscopy

Citation
A. Midelfart et al., Detection of dexamethasone in the cornea and lens by NMR spectroscopy, GR ARCH CL, 237(5), 1999, pp. 415-423
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Optalmology
Journal title
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
ISSN journal
0721-832X → ACNP
Volume
237
Issue
5
Year of publication
1999
Pages
415 - 423
Database
ISI
SICI code
0721-832X(199905)237:5<415:DODITC>2.0.ZU;2-0
Abstract
Background: To study the penetration and metabolism of dexamethasone phosph ate in the cornea and lens from rabbit following topical administration. Methods: After topical administration of 0.1, 1 and 10% dexamethasone phosp hate solutions, respectively, the cornea and lens were removed and extracte d with either perchloric acid (PCA) or with chloroform/methanol to study th e lipophilic and hydrophilic metabolites of the drug. The extracts were ana lyzed with H-1 and F-19 NMR spectroscopy. Results: Using F-19 NMR spectroscopy, both dexamethasone and dexamethasone phosphate were detected simultaneously in the extract of the cornea and len s obtained from the eyes denuded of the epithelium before the treatment. Us ing 10 and 1% drug solutions, the signals from dexamethasone were also dete ctable in the H-1 NMR spectra among many endogenous metabolites. Conclusion: H-1 and F-19 NMR spectroscopy was shown to be a useful method i n the study of penetration and metabolism of dexamethasone in the eye, part icularly because both the lipophilic and hydrophilic metabolites of the dru g could be detected simultaneously.