Differentiation of Vibrio alginolyticus strains isolated from sardinian waters by ribotyping and a new rapid PCR fingerprinting method

Citation
S. Zanetti et al., Differentiation of Vibrio alginolyticus strains isolated from sardinian waters by ribotyping and a new rapid PCR fingerprinting method, APPL ENVIR, 65(5), 1999, pp. 1871-1875
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
0099-2240 → ACNP
Volume
65
Issue
5
Year of publication
1999
Pages
1871 - 1875
Database
ISI
SICI code
0099-2240(199905)65:5<1871:DOVASI>2.0.ZU;2-Z
Abstract
We investigated the usefulness of a novel PCR fingerprinting technique, bas ed on the specific amplification of genomic regions, to differentiate 30 Vi brio alginolyticus strains isolated in Sardinian waters. The different prof iles obtained were scanned and analyzed by a computer program in order to d etermine genetic relationships. The results were then compared with the pat terns obtained by ribotyping with HindIII, KpnI, and XbaI restriction enzym es. PCR fingerprinting could differentiate the strains analyzed into 12 dif ferent patterns, whereas ribotyping with XbaI, which produced the highest n umber of patterns, generated only 7 different profiles. This study revealed the superior discriminative power of the proposed technique for the differ entiation of related V. alginolyticus strains and the potential use of PCR fingerprinting in epidemiological studies.