The affinity of lipid-coated microbubbles to maturing spinal cord injury sites

Citation
Iu. Kureshi et al., The affinity of lipid-coated microbubbles to maturing spinal cord injury sites, NEUROSURGER, 44(5), 1999, pp. 1047-1053
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurology,"Neurosciences & Behavoir
Journal title
NEUROSURGERY
ISSN journal
0148-396X → ACNP
Volume
44
Issue
5
Year of publication
1999
Pages
1047 - 1053
Database
ISI
SICI code
0148-396X(199905)44:5<1047:TAOLMT>2.0.ZU;2-R
Abstract
OBJECTIVE: This laboratory has demonstrated that lipid-coated microbubbles (LCMs) effectively aggregate and deliver chemotherapeutic drugs into rat br ain tumor cells and antigliosis agents into maturing rat brain injury sites . In this study, we report the affinity of tail vein-injected LCMs to the i njured rat spinal cord by a compressive lesion to the upper thoracic region . METHODS: The accumulation of LCMs in the injured spinal cord was analyzed b y labeling it with a lipid-soluble fluorescent dye, 3,3'-dioctadecyloxacarb ocyanine perchlorate. Indices of glial fibrillary acidic protein were measu red concomitantly with 3,3'-dioctadecyloxacarbocyanine perchlorate-labeled LCMs using confocal microscopy. RESULTS: There was no aggregation of LCMs accumulated 1 and 6 hours after i njury; however, when given 2, 4, and 7 days after injury, LCMs showed a cle ar affinity for the injured region. LCM aggregation shifted from the centra l necrotic area of the injury on postinjury Day 2 and postinjury Day 4 to t he white matter among glial fibrillary acidic protein-positive astrocytes b y postinjury Day 7. CONCLUSION: Affinity of LCMs for spinal cord injury sites may be mediated i n the early stages after injury by proliferating macrophages in the necroti c center, and then in later stages by glial fibrillary acidic protein-posit ive astrocytes in adjacent white matter. These findings suggest a potential for using LCMs as a delivery vehicle to concentrate lipid-soluble agents i n spinal cord injury sites.