Perinatal sidestream cigarette smoke exposure and the developing pulmonarysurfactant system in rats

Citation
S. Subramaniam et al., Perinatal sidestream cigarette smoke exposure and the developing pulmonarysurfactant system in rats, HUM EXP TOX, 18(4), 1999, pp. 206-211
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
HUMAN & EXPERIMENTAL TOXICOLOGY
ISSN journal
0960-3271 → ACNP
Volume
18
Issue
4
Year of publication
1999
Pages
206 - 211
Database
ISI
SICI code
0960-3271(199904)18:4<206:PSCSEA>2.0.ZU;2-R
Abstract
1 Epidemiological studies have strongly implicated passive smoking with inc reased incidence of various respiratory diseases in children. Our earlier s tudies have shown that chronic exposure to tobacco smoke significantly chan ges the composition and the surface activity of the pulmonary surfactant in adult rats. The aim of the present study was to determine if perinatal exp osure to sidestream cigarette smoke influences the composition and function of pulmonary surfactant system in developing rat pups. 2 Pregnant Sprague Dawley rats were exposed to sidestream cigarette smoke i n a whole body exposure chamber for a total of 6 h each day and the pups bo rn to these dams were further exposed to sidestream smoke for 2 h/day durin g postnatal period. Exposure of animals to smoke was ascertained by measuri ng their plasma cotinine. Surfactant analysis was performed on bronchoalveo lar lavage fluids (BALF) collected from pups on postnatal day 1, 3, 7, 14, 21 and 35. The phospholipid (PL) content, percentage disaturated phosphatid ylcholine (DSPC) and surfactant proteins (SP-A and SP-B) in BALF surfactant preparations from sham and smoke-exposed pups were compared. 3 Significant differences between the two groups were observed at two expos ure points: a reduced level of SPA on day 1, and, a higher level of SP-A an d PLs on day 21, in smoke-exposed pups. Surface activity analysis of the su rfactant films by pulsating bubble surfactometer did not show any significa nt differences between the sham and smoke-exposed groups at any exposure po int. 4 The results indicate that perinatal exposure to sidestream smoke is capab le of producing biochemical changes in the composition of pulmonary surfact ant at different stages of development but these changes do not influence s urface tension reducing properties of the surfactant.