Nucleotide structure and characterization of the murine gene encoding the endothelial cell protein C receptor

Citation
Z. Liang et al., Nucleotide structure and characterization of the murine gene encoding the endothelial cell protein C receptor, THROMB HAEM, 81(4), 1999, pp. 585-588
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cardiovascular & Hematology Research
Journal title
THROMBOSIS AND HAEMOSTASIS
ISSN journal
0340-6245 → ACNP
Volume
81
Issue
4
Year of publication
1999
Pages
585 - 588
Database
ISI
SICI code
0340-6245(199904)81:4<585:NSACOT>2.0.ZU;2-J
Abstract
The nucleotide sequence of the entire gene encoding the murine endothelial cell receptor for activated protein C (EPCR) has been determined. A total o f 5303 bp of DNA was sequenced that included 4 exons and three introns, whi ch constituted the coding region of the gene, as well as 393 bp upstream of the first exon and 841 bp downstream of the last exon, From the locations of the introns in this gene and analysis of the exon structures, it is clea r the EPCR gene is a member of the CD1 class of multiple histocompatibility proteins, and its cDNA sequence is nearly identical to that of CCD41, a ce ntrosome-associated protein. All elements needed for RNA polymerase II-base d transcription are predicted to exist in the 5' uncoded region of the gene , and potential 3' regulatory sequences for efficient polyadenylation have been located at their optimal locations. A variety of highly probable trans cription factor binding sites have been located in the 5' region of the gen e, These data suggest that the EPCR gene is under efficient transcriptional control, and support the finding that this gene product may be involved in the inflammatory pathway.