THE EFFECT OF LP3 ENLARGEMENT ON THE FOLDING AND CATALYSIS OF HEPATITIS-DELTA VIRUS CIS-CLEAVING RIBOZYME

Citation
Hn. Wu et al., THE EFFECT OF LP3 ENLARGEMENT ON THE FOLDING AND CATALYSIS OF HEPATITIS-DELTA VIRUS CIS-CLEAVING RIBOZYME, FEBS letters, 439(3), 1998, pp. 312-316
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,"Cell Biology",Biophysics
Journal title
ISSN journal
0014-5793
Volume
439
Issue
3
Year of publication
1998
Pages
312 - 316
Database
ISI
SICI code
0014-5793(1998)439:3<312:TEOLEO>2.0.ZU;2-P
Abstract
Oligonucleotides were inserted into the Lp3 region of a hepatitis delt a virus (HDV) cis-cleaving ribozyme and the effect of Lp3 enlargement on ribozyme folding was examined by assaying the activity of each muta nt. The location of insertion and the sequence of the inserted oligonu cleotide had distinct effects on ribozyme activity, and the HDV ribozy me was capable of adopting the active structure for cis-cleavage when a pentanucleotide was inserted into the 3' portion of Lp3, Furthermore , whether the insertion mutant cis-cleaved or not, the structure of Lp 3 was altered by the inserted oligonucleotide, These findings disclose that the 5' rather than the 3' portion of Lp3 is critical for ribozym e folding and catalysis. (C) 1998 Federation of European Biochemical S ocieties.