FUNCTIONAL IDENTIFICATION OF A FATTY-ACID DELTA-5 DESATURASE GENE FROM CAENORHABDITIS-ELEGANS

Citation
Lv. Michaelson et al., FUNCTIONAL IDENTIFICATION OF A FATTY-ACID DELTA-5 DESATURASE GENE FROM CAENORHABDITIS-ELEGANS, FEBS letters, 439(3), 1998, pp. 215-218
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,"Cell Biology",Biophysics
Journal title
ISSN journal
0014-5793
Volume
439
Issue
3
Year of publication
1998
Pages
215 - 218
Database
ISI
SICI code
0014-5793(1998)439:3<215:FIOAFD>2.0.ZU;2-G
Abstract
We have identified a cDNA from the nematode worm Caenorhabditis elegan s that encodes a fatty acid Delta(5) desaturase, Saccharomyces cerevis iae expressing the full-length cDNA was able to convert di-homo-gamma- linolenic acid to arachidonic acid, thus confirming Delta(5) desaturat ion. The 1341 hp Delta(5) desaturase sequence contained an N-terminal cytochrome b(5) domain and was located within a kilobase of the C. ele gans Delta(6) desaturase on chromosome IV. With an amino acid identity of 45% it is possible that one of these genes arose from the other by gene duplication. This is the first example of a Delta(5) desaturase gene isolated from an animal. (C) 1998 Federation of European Biochemi cal Societies.