EVIDENCE FOR THE PARTICIPATION OF THE NEURON-SPECIFIC CDK5 ACTIVATOR P35 DURING LAMININ-ENHANCED AXONAL GROWTH

Citation
G. Paglini et al., EVIDENCE FOR THE PARTICIPATION OF THE NEURON-SPECIFIC CDK5 ACTIVATOR P35 DURING LAMININ-ENHANCED AXONAL GROWTH, The Journal of neuroscience, 18(23), 1998, pp. 9858-9869
Citations number
59
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
0270-6474
Volume
18
Issue
23
Year of publication
1998
Pages
9858 - 9869
Database
ISI
SICI code
0270-6474(1998)18:23<9858:EFTPOT>2.0.ZU;2-W
Abstract
Cultures of cerebellar macroneurons were used to study the pattern of expression, subcellular localization, and function of the neuronal cdk 5 activator p35 during laminin-enhanced axonal growth. The results obt ained indicate that laminin, an extracellular matrix molecule capable of selectively stimulating axonal extension and promoting MAP1B phosph orylation at a proline-directed protein kinase epitope, selectively st imulates p35 expression, increases its association with the subcortica l cytoskeleton, and accelerates its redistribution to the axonal growt h cones. Besides, suppression of p35, but not of a highly related isof orm designated as p39, by antisense oligonucleotide treatment selectiv ely reduces cdk5 activity, laminin-enhanced axonal elongation, and MAP 1b phosphorylation. Taken collectively, the present results suggest th at cdk5/p35 may serve as an important regulatory linker between enviro nmental signals (e.g., laminin) and constituents of the intracellular machinery (e.g., MAP1B) involved in axonal elongation.