EPIDERMAL GROWTH-FACTOR UP-REGULATES AMINOPEPTIDASE N AND 5'-NUCLEOTIDASE IN HUMAN GLOMERULAR MESANGIAL CELLS

Citation
V. Stefanovic et al., EPIDERMAL GROWTH-FACTOR UP-REGULATES AMINOPEPTIDASE N AND 5'-NUCLEOTIDASE IN HUMAN GLOMERULAR MESANGIAL CELLS, Kidney & blood pressure research, 21(5), 1998, pp. 310-316
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Peripheal Vascular Diseas","Urology & Nephrology",Physiology
ISSN journal
1420-4096
Volume
21
Issue
5
Year of publication
1998
Pages
310 - 316
Database
ISI
SICI code
1420-4096(1998)21:5<310:EGUANA>2.0.ZU;2-A
Abstract
Because epidermal growth factor (EGF) is likely to be released in the glomeruli during glomerular injury and mesangial cells possess specifi c receptors for EGF we thought it to be of interest to examine whether this growth factor could influence the expression of ectoenzymes in c ultured human mesangial cells. EGF stimulated 5'-nucleotidase and amin opeptidase N activities in intact human mesangial cells in a time- (24 -72 h) and dose-dependent (0.1-50 ng.ml(-1)) manner. Maximum stimulati on represented 2.7- and 2-fold basal activities for 5'-nucleotidase an d aminopeptidase N, respectively. EGF did not influence cyclic AMP pro duction, and its effect on 5'-nucleotidase was additive to that of for skolin or 8-bromo-cAMP. In contrast, genistein (10 mg.ml(-1)), an inhi bitor of tyrosine kinase, prevented EGF-dependent stimulation of amino peptidase N and 5'-nucleotidase, suggesting that protein phosphorylati on was involved in the signaling mechanism. EGF stimulated specificall y the latter two enzymes since it had no effect on other ectoenzymes i ncluding alkaline phosphodiesterase I and Mg2+-ATPase activities. Thes e results demonstrate that EGF via the control of 5'-nucleotidase and aminopeptidase N, which are implied in adenosine formation and peptide processing, respectively, could play a role in human cultured mesangi al cell contractility and proliferation.