CHANGES IN THE CYTOSKELETON ACCOMPANYING INFECTION-INDUCED NUCLEAR-MOVEMENTS AND THE HYPERSENSITIVE RESPONSE IN PLANT-CELLS INVADED BY RUSTFUNGI

Citation
D. Skalamera et Mc. Heath, CHANGES IN THE CYTOSKELETON ACCOMPANYING INFECTION-INDUCED NUCLEAR-MOVEMENTS AND THE HYPERSENSITIVE RESPONSE IN PLANT-CELLS INVADED BY RUSTFUNGI, Plant journal, 16(2), 1998, pp. 191-200
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences",Biology
Journal title
ISSN journal
0960-7412
Volume
16
Issue
2
Year of publication
1998
Pages
191 - 200
Database
ISI
SICI code
0960-7412(1998)16:2<191:CITCAI>2.0.ZU;2-2
Abstract
During the infection of cowpea (Vigna unguiculata) by the cowpea rust fungus (Uromyces vignae, race 1) the plant nucleus moves towards and a way from the invading hypha and eventually moves close to the fungus i n the susceptible cultivar while it remains away in two cultivars whic h subsequently respond by resistance gene-dependent plant cell death ( the hypersensitive response, HR). The role of plant cytoskeleton in th ese responses was investigated by fluorescent microscopy and treatment s with anticytoskeletal drugs. Observations of microtubule organizatio n prior to cell death revealed that the sequence of events leading to protoplast collapse differed between the two resistant cultivars, sugg esting a possibility of multiple pathways for cellular degradation dur ing the HR. Different fixations produced two different microfilament p atterns: a filament network and cables. Microfilament network remained visible even at later stages of cell death. Oryzalin and taxol reduce d the incidence of autofluorescence that develops late in the death pr ocess, indicating a role of microtubules in the deposition of phenolic s by adjacent living cells. Cell death and nuclear movements were not affected by oryzalin and taxol but were inhibited by cytochalasin E, s uggesting that the microfilaments are required for the HR.