LIPASE FROM PSEUDOMONAS-AERUGINOSA LP602 - BIOCHEMICAL-PROPERTIES ANDAPPLICATION FOR WASTE-WATER TREATMENT

Citation
S. Dharmsthiti et B. Kuhasuntisuk, LIPASE FROM PSEUDOMONAS-AERUGINOSA LP602 - BIOCHEMICAL-PROPERTIES ANDAPPLICATION FOR WASTE-WATER TREATMENT, Journal of industrial microbiology & biotechnology, 21(1-2), 1998, pp. 75-80
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biothechnology & Applied Migrobiology
Volume
21
Issue
1-2
Year of publication
1998
Pages
75 - 80
Database
ISI
SICI code
Abstract
Lipase from Pseudomonas aeruginosa LP602, a bacterial strain isolated from a domestic wastewater sample, was preliminarily characterized. Th e enzyme exhibited maximum lipolytic activity at pH 8.0 where it was a lso stably maintained. At 55 degrees C, the lipase had the highest act ivity but not stability. The enzyme was insensitive to EDTA and to man y ions tested except Zn2+. It was sensitive to SDS but not to Tween-20 , Tween-80 or Triton X-100, The enzyme was active towards a number of commercial food grade fats and oils. A suitable medium formula for lip ase production was MMP containing 6.25% whey as a carbon source, 1% so ybean oil as inducer and 0.5% yeast extract supplement. The culture wa s fed with glucose to a final concentration of 0.1% at the 15th hour o f incubation. Lipase production under this condition was 3.5 U ml(-1). Both P. aeruginosa LP602 cells and the lipase were shown to be usable for lipid-rich wastewater treatment.