S. Dharmsthiti et B. Kuhasuntisuk, LIPASE FROM PSEUDOMONAS-AERUGINOSA LP602 - BIOCHEMICAL-PROPERTIES ANDAPPLICATION FOR WASTE-WATER TREATMENT, Journal of industrial microbiology & biotechnology, 21(1-2), 1998, pp. 75-80
Lipase from Pseudomonas aeruginosa LP602, a bacterial strain isolated
from a domestic wastewater sample, was preliminarily characterized. Th
e enzyme exhibited maximum lipolytic activity at pH 8.0 where it was a
lso stably maintained. At 55 degrees C, the lipase had the highest act
ivity but not stability. The enzyme was insensitive to EDTA and to man
y ions tested except Zn2+. It was sensitive to SDS but not to Tween-20
, Tween-80 or Triton X-100, The enzyme was active towards a number of
commercial food grade fats and oils. A suitable medium formula for lip
ase production was MMP containing 6.25% whey as a carbon source, 1% so
ybean oil as inducer and 0.5% yeast extract supplement. The culture wa
s fed with glucose to a final concentration of 0.1% at the 15th hour o
f incubation. Lipase production under this condition was 3.5 U ml(-1).
Both P. aeruginosa LP602 cells and the lipase were shown to be usable
for lipid-rich wastewater treatment.