ACID-BASE RESPONSE OF BACTERIAL SUSPENSIONS

Citation
N. Rius et al., ACID-BASE RESPONSE OF BACTERIAL SUSPENSIONS, Journal of industrial microbiology & biotechnology, 21(1-2), 1998, pp. 65-74
Citations number
32
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biothechnology & Applied Migrobiology
Volume
21
Issue
1-2
Year of publication
1998
Pages
65 - 74
Database
ISI
SICI code
Abstract
The response of suspensions of non-proliferating bacteria to external pH ranging from 3.6 to 9.9 was examined. The acid-base response is aff ected by the number of viable cells in the suspensions and culture con ditions (aerobiosis or anaerobiosis) but not by culture time, composit ion of the culture medium or the age of suspensions. The results with carbonylcyanide m-chlorophenylhydrazone-and N, N'- dicyclohexylcarbodi imide-treated cells indicated that passive proton conductance and prot on translocation by ATP could be involved in this response, pH profile s of suspensions of 18 chemoheterotrophic Gram-negative and Gram-posit ive bacteria support the hypothesis that the proton gradient generated by an alkali or an acid shift could be dissipated by bacteria with re spiratory metabolism.