A MUTANT STRAIN OF LEUCONOSTOC-MESENTEROIDES B-1355 PRODUCING A GLUCOSYLTRANSFERASE SYNTHESIZING ALPHA(1-]2) GLUCOSIDIC LINKAGES

Citation
Mr. Smith et al., A MUTANT STRAIN OF LEUCONOSTOC-MESENTEROIDES B-1355 PRODUCING A GLUCOSYLTRANSFERASE SYNTHESIZING ALPHA(1-]2) GLUCOSIDIC LINKAGES, Journal of industrial microbiology & biotechnology, 21(1-2), 1998, pp. 37-45
Citations number
32
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biothechnology & Applied Migrobiology
Volume
21
Issue
1-2
Year of publication
1998
Pages
37 - 45
Database
ISI
SICI code
Abstract
A mutant strain (R1510) of Leuconostoc mesenteroides 8-1355 was isolat ed which synthesized primarily an insoluble polysaccharide and little soluble polysaccharide when grown in sucrose-containing medium, Glucos e or sucrose cultures of this strain produced a single intense band of GTF-1 activity of 240 kDa on SDS gels, and a number of faint, smaller bands. Oligosaccharides synthesized by strain R1510 from methyl-alpha -D-glucoside and sucrose included a trisaccharide whose structure cont ained an alpha(1 --> 2) glucosidic linkage, This type of linkage has n ot been seen before in any products from strain 8-1355 or its mutant d erivatives, The structure of the purified trisaccharide was confirmed by C-13-nuclear magnetic resonance. The insoluble polysaccharide also contained alpha(1 --> 2) branch linkages, as determined by methylation analysis, showing that synthesis of the linkages was not peculiar to methyl-alpha-D-glucoside, GTF-1, which had been excised with a razor b lade from an SDS gel of a culture of the parent strain 8-1355, produce d the same trisaccharides as strain R1510, showing that GTF-1 from the wild-type strain was the same as GTF-1 from strain R1510, Mutant stra ins resembling strain R1510, but producing a single intense band of al ternansucrase (200 kDa) instead of GTF-1 were also isolated, suggestin g that mutations may be generated which diminished the activities for any two of the three GTFs of strain 81355 relative to the third, Strai n R1554 produced a soluble form of alternansucrase, while strain R1588 produced a cell-associated form. The mechanism(s) by which specific G TFs become associated with the cells of L. mesenteroides was not explo red.