CONVERSION OF SUCROSE TO ISOMALTULOSE BY KLEBSIELLA-PLANTICOLA CCRC-19112

Citation
Jh. Huang et al., CONVERSION OF SUCROSE TO ISOMALTULOSE BY KLEBSIELLA-PLANTICOLA CCRC-19112, Journal of industrial microbiology & biotechnology, 21(1-2), 1998, pp. 22-27
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biothechnology & Applied Migrobiology
Volume
21
Issue
1-2
Year of publication
1998
Pages
22 - 27
Database
ISI
SICI code
Abstract
An isomaltulose-producing bacterium was isolated and taxonomically cha racterized. Its morphological and biochemical properties conform best to those described for Klebsiella planticola, When cultured under opti mal conditions, the organism simultaneously converted sucrose into bot h isomaltulose (alpha-D-glucopyranosyl-1,6-fructose) and trehalulose ( alpha-D-glucopyranosyl-1,1-fructose) with substrate conversion rates o f 80% and 15%, respectively. Sucrose and Bacto-tryptone were the most effective carbon and supplemental nitrogen sources, respectively, for producing cells of high isomaltulose-forming ability. None of several inorganic salts tested had any significant effect, The major product f ormed in the reaction mixture was verified to be isomaltulose by co-ch romatography and IR spectroscopy.