PRODUCTION OF RECOMBINANT HIRUDIN IN HANSENULA-POLYMORPHA - VARIATIONOF GENE-EXPRESSION LEVEL DEPENDS ON METHANOL OXIDASE AND FERMENTATIONSTRATEGIES

Citation
Ch. Kim et al., PRODUCTION OF RECOMBINANT HIRUDIN IN HANSENULA-POLYMORPHA - VARIATIONOF GENE-EXPRESSION LEVEL DEPENDS ON METHANOL OXIDASE AND FERMENTATIONSTRATEGIES, Journal of industrial microbiology & biotechnology, 21(1-2), 1998, pp. 1-5
Citations number
13
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biothechnology & Applied Migrobiology
Volume
21
Issue
1-2
Year of publication
1998
Pages
1 - 5
Database
ISI
SICI code
Abstract
Hansenula polymorpha strains were developed and compared for their lev el of expression of the anticoagulant hirudin, H. polymorpha DL1-57 ha rboring an autonomously replicating sequence, HARS36, efficiently expr essed the gene for recombinant hirudin, The effect of methanol oxidase (MOX) on the expression of the hirudin gene in H. polymorpha DL1-57 w as studied, and the fermentation strategies coupled with the MOX activ ity and an antioxidant, tocopherol, were also examined.