REGULATED EXPRESSION OF THE INTEGRIN ALPHA-9-BETA-1 IN THE EPITHELIUMOF THE DEVELOPING HUMAN GUT AND IN INTESTINAL-CELL LINES - RELATION WITH CELL-PROLIFERATION

Citation
N. Desloges et al., REGULATED EXPRESSION OF THE INTEGRIN ALPHA-9-BETA-1 IN THE EPITHELIUMOF THE DEVELOPING HUMAN GUT AND IN INTESTINAL-CELL LINES - RELATION WITH CELL-PROLIFERATION, Journal of cellular biochemistry, 71(4), 1998, pp. 536-545
Citations number
48
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,"Cell Biology
ISSN journal
0730-2312
Volume
71
Issue
4
Year of publication
1998
Pages
536 - 545
Database
ISI
SICI code
0730-2312(1998)71:4<536:REOTIA>2.0.ZU;2-P
Abstract
The integrin alpha 9 beta 1 is one of the recently identified integrin s whose expression is restricted to specialized tissues. Its exact fun ction is still unknown. In the present study, we have analyzed the exp ression of the alpha 9 subunit in human fetal and adult small intestin al and colonic epithelia as well as in intestinal cell lines by indire ct immunofluorescence, immunoprecipitation, Western blot, and Northern blot. In intact tissues, the antigen was restricted to the basolatera l domain of epithelial cells in intestinal crypts at the fetal stage a nd was absent in the adult. The alpha 9 beta 1 integrin was also detec ted in the intestinal cell lines HIEC-6 and Caco-2/15. The presence of alpha 9 beta 1 in HIEC-6 was found to be consistent with their prolif erative crypt-like status. In Caco-2/15 cells, the integrin was presen t at high levels in proliferating cells but was downregulated when cel ls cease to grow and undertake their differentiation. EGF treatment, w h ich is known to maintain Caco-2/15 cells in a proliferative state, r esulted in higher levels of alpha 9 as compared to control cells. Take n together, these observations suggest a relation between integrin alp ha 9 beta 1 expression and proliferation in human intestinal cells. (C ) 1998 Wiley-Liss, Inc.