PARALLEL PRODUCTION OF OLIGONUCLEOTIDE ARRAYS USING MEMBRANES AND REAGENT JET PRINTING

Citation
Di. Stimpson et al., PARALLEL PRODUCTION OF OLIGONUCLEOTIDE ARRAYS USING MEMBRANES AND REAGENT JET PRINTING, BioTechniques, 25(5), 1998, pp. 886-890
Citations number
18
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,"Biochemical Research Methods
Journal title
BioTechniques → ACNP
ISSN journal
0736-6205
Volume
25
Issue
5
Year of publication
1998
Pages
886 - 890
Database
ISI
SICI code
0736-6205(1998)25:5<886:PPOOAU>2.0.ZU;2-V
Abstract
Here, we present a low-cost method to produce compact arrays using mic roporous materials and reagent jetting. Oligonucleotides are immobiliz ed on membrane sheets as a series of lines. The membrane sheer is then rolled and bound, and the spiral structure is cut like a ''jelly roll '' to produce identical arrays. The spiral arrays behave much like lar ger formats using membranes, and hybridization detection can be accomp lished using standard signal-generation mechanisms. The method is part icularly useful for producing identical arrays from pre-synthesized ol igonucleotides.