CELL-SPECIFIC ECDYSONE-INDUCIBLE EXPRESSION OF FLP RECOMBINASE IN MAMMALIAN-CELLS

Citation
Ja. Sawicki et al., CELL-SPECIFIC ECDYSONE-INDUCIBLE EXPRESSION OF FLP RECOMBINASE IN MAMMALIAN-CELLS, BioTechniques, 25(5), 1998, pp. 868
Citations number
13
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,"Biochemical Research Methods
Journal title
BioTechniques → ACNP
ISSN journal
0736-6205
Volume
25
Issue
5
Year of publication
1998
Database
ISI
SICI code
0736-6205(1998)25:5<868:CEEOFR>2.0.ZU;2-L
Abstract
The ability of site-specific recombinases, like FLP and Cre, to cataly ze alterations in genomic DNA is well established, whereas their appli cation to genetic engineering strategies has been restricted because o f the inability to temporally regulate their expression and subsequent recombination events in specific populations of cells. We describe a regulatory system for ecdysone-controlled expression of FLP recombinas e. Furthermore, we demonstrate that ecdysone-induced, FLP-mediated sit e-specific recombination events can be targeted to specific cells. Thi s system can be applied to cell-lineage studies as well as to the desi gn of gene-therapy strategies, particularly in stem cells.