COHERENT BACKSCATTER ENHANCES REFLECTION CONFOCAL MICROSCOPY

Citation
Ch. Keith et al., COHERENT BACKSCATTER ENHANCES REFLECTION CONFOCAL MICROSCOPY, BioTechniques, 25(5), 1998, pp. 858
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,"Biochemical Research Methods
Journal title
BioTechniques → ACNP
ISSN journal
0736-6205
Volume
25
Issue
5
Year of publication
1998
Database
ISI
SICI code
0736-6205(1998)25:5<858:CBERCM>2.0.ZU;2-5
Abstract
When thin optically transparent specimens are grown on reflective subs trates, contrast in reflection confocal microscopy is markedly enhance d. This enhanced contrast allows for the visualization of the thin fil opodia and organelles contained within the neuritic processes of PC12 cells in culture. The characteristics of this contrast enhancement sug gest that it arises because of interference between light scattered fr om rile specimen and coherently backscattered illumination reflected o ff the substrate. This technique provides a method for visualizing liv ing cells or other similarly transparent objects on opaque substrates in a nondestructive manner.