MYOCARDIAL TISSUE ENGINEERING WITH AUTOLOGOUS MYOBLAST IMPLANTATION

Citation
J. Dorfman et al., MYOCARDIAL TISSUE ENGINEERING WITH AUTOLOGOUS MYOBLAST IMPLANTATION, Journal of thoracic and cardiovascular surgery, 116(5), 1998, pp. 744-751
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cardiac & Cardiovascular System",Surgery
ISSN journal
0022-5223
Volume
116
Issue
5
Year of publication
1998
Pages
744 - 751
Database
ISI
SICI code
0022-5223(1998)116:5<744:MTEWAM>2.0.ZU;2-R
Abstract
Objective: Implanting myoblasts derived from autologous skeletal muscl e, that is, satellite cells, for myocardial replacement has many advan tages when compared with implanting either fetal cardiac myocytes (eth ical and donor availability issues) or established cell lines (oncogen icity), Furthermore, autologous myoblasts do not require immunosuppres sion. The feasibility of satellite cell differentiation into muscle fi bers, after implantation into the myocardium, was confirmed by means o f a unique cell-labeling technique. Methods: Myoblasts (satellite cell s) isolated from the skeletal muscle of adult rats are labeled with 4' ,6-diamidino-2-phenylindone, which binds to DNA and to the protein tub ulin to form a fluorescent complex, and implanted into the left ventri cular wall of isogenic rats. The specimens are harvested I to 4 weeks after myoblast implantation. Histologic sections are examined under a fluorescent microscope. Results: The labeling efficiency of satellite cells with 4',6-diamidino-2-phenylindole is nearly 100%. Tn 4 specimen s, the progressive differentiation of implanted myoblasts into fully d eveloped striated muscle fibers can be observed. Conclusion: Our earli er studies of autologous myoblast implantation into the cryoinjured my ocardium of dogs suggested that these cells could differentiate into c ardiac myocytes. However, it had been difficult to firmly establish th ese Findings with the use of cell markers, thereby proving that the ne omyocardium had indeed been derived from the implanted myoblasts, In t his study, using 4',6-diamidino-2-phenylindole as a satellite cell mar ker, we were able to demonstrate that the implanted satellite cells di d in fact differentiate into fully developed, labeled muscle fibers. B ecause of the obvious advantages of using autologous donor myoblasts, the clinical application of this approach may provide a novel strategy for the future management of heart failure.