LOCATION OF FLUOROTRYPTOPHAN SEQUESTERED IN AN AMPHIPHILIC NANOPARTICLE BY ROTATIONAL-ECHO DOUBLE-RESONANCE NMR

Citation
Ah. Baugher et al., LOCATION OF FLUOROTRYPTOPHAN SEQUESTERED IN AN AMPHIPHILIC NANOPARTICLE BY ROTATIONAL-ECHO DOUBLE-RESONANCE NMR, Biophysical journal, 75(5), 1998, pp. 2574-2576
Citations number
13
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biophysics
Journal title
ISSN journal
0006-3495
Volume
75
Issue
5
Year of publication
1998
Pages
2574 - 2576
Database
ISI
SICI code
0006-3495(1998)75:5<2574:LOFSIA>2.0.ZU;2-S
Abstract
Rotational-echo double-resonance (REDOR) C-13 NMR spectra (with F-19 d ephasing) have been obtained of 6-fluorotryptophan complexed by a poly meric amphiphilic nanosphere consisting of a polystyrene core covalent ly attached to a poly(acrylic acid)-polyacrylamide shell. The REDOR sp ectra show that aromatic carbons from the polystyrene core and oxygena ted carbons in the poly(acrylic acid)-polyacrylamide shell are both pr oximate to the F-19 of 6-fluorotryptophan. Molecular modeling restrain ed by distances inferred from the REDOR spectra suggests that all of t he 6-fluorotryptophans are in the shell but within 10 Angstrom of the core-shell interface.