CONSTRUCTION OF HUMAN INSULIN GENE-EXPRESSION RECOMBINANT AND ITS EFFECT ON BLOOD-GLUCOSE OF DIABETIC RATS

Citation
H. Wang et al., CONSTRUCTION OF HUMAN INSULIN GENE-EXPRESSION RECOMBINANT AND ITS EFFECT ON BLOOD-GLUCOSE OF DIABETIC RATS, Chinese medical journal, 111(10), 1998, pp. 899-902
Citations number
9
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medicine, General & Internal
Journal title
ISSN journal
0366-6999
Volume
111
Issue
10
Year of publication
1998
Pages
899 - 902
Database
ISI
SICI code
0366-6999(1998)111:10<899:COHIGR>2.0.ZU;2-7
Abstract
Objective To study the effect of human insulin gene modified fibroblas ts on blood glucose in diabetic rats. Methods An expression vector con taining human insulin gene was constructed by recombinant DNA techniqu e and introduced into fibroblast Ltk(-) cells by lipofectin-mediated D NA transfection. Following G418 screening, the survived cells were sel ected and enriched. Finally a cell line, PRI-12 was generated for the highest insulin production. Then, these cells were injected into strep tozocin (STZ)-induced diabetic rats. Results insulin DNA transfected L tk(-) cells were able to express insulin at a high level in a long-ter m culture. Furthermore, these Ltk(-) transfectants could decrease bloo d glucose significantly (P < 0.01) and obviously increase the body wei ght (P < 0.01) when they were injected into STZ-induced diabetic rats. Conclusions These results suggest that the cell lines transfected ins ulin gene could secrete insulin and execute effect on diabetic rats. T he study provides support for the view that somatic cell gene therapy offers a potential approach to delivery insulin into diabetes mellitus .