REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY-METHOD FOR QUANTITATING 6-MERCAPTOPURINE AND ITS METHYLATED AND NONMETHYLATED METABOLITES IN A SINGLE-SAMPLE

Citation
H. Mawatari et al., REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY-METHOD FOR QUANTITATING 6-MERCAPTOPURINE AND ITS METHYLATED AND NONMETHYLATED METABOLITES IN A SINGLE-SAMPLE, Journal of chromatography B. Biomedical sciences and applications, 716(1-2), 1998, pp. 392-396
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
ISSN journal
1387-2273 → ACNP
Volume
716
Issue
1-2
Year of publication
1998
Pages
392 - 396
Database
ISI
SICI code
0378-4347(1998)716:1-2<392:RHLAF>2.0.ZU;2-6
Abstract
Methods of assaying 6-mercaptopurine (6MP) and its methylated and non- methylated metabolites are essential for the therapeutic dose in treat ing patients with acute lymphoblastic leukemia. However, previous meth ods are technically complicated and unsuitable for clinical use. Thus, we have now developed a method utilizing reversed-phase highperforman ce liquid chromatography (HPLC) in order to quantify these compounds i n human red blood cells (RBCs) in a single sample to serve as an index of cytotoxic activity. The agents 6MP 6-thioguanine (6TG) and 6-methy lmercaptopurine (6MMP) were well separated by this assay. Linear relat ionships were observed between the peak areas and the RBC concentratio ns of 6MP, 6TG and 6MMP over the range of 20-2000, 18-1800 and 18-1800 pmol per 25 mg hemoglobin (Hb), respectively. The limit of quantitati on of the assay is 20, 18 and 18 pmol per 25 mg Hb, respectively. This assay system is suitable for routine clinical use. (C) 1998 Elsevier Science B.V. All rights reserved.