SIMULTANEOUS DETERMINATION OF NICOTINIC-ACID AND ITS METABOLITES IN RAT URINE BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY WITH PHOTODIODE AWAY DETECTION

Citation
M. Iwaki et al., SIMULTANEOUS DETERMINATION OF NICOTINIC-ACID AND ITS METABOLITES IN RAT URINE BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY WITH PHOTODIODE AWAY DETECTION, Journal of chromatography B. Biomedical sciences and applications, 716(1-2), 1998, pp. 335-342
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
ISSN journal
1387-2273 → ACNP
Volume
716
Issue
1-2
Year of publication
1998
Pages
335 - 342
Database
ISI
SICI code
0378-4347(1998)716:1-2<335:SDONAI>2.0.ZU;2-Q
Abstract
Nicotinic acid, nicotinamide and their possible metabolites were succe ssfully separated within 17 min by micellar electrokinetic chromatogra phy using 50 mM berate buffer (pH 9.0) containing 150 mM sodium dodecy l sulfate as the running buffer. Calibration curves for all compounds showed good linearity in a range of 5 mu g/ml and 250 mu g/ml with goo d correlation. The present method did not require any clean-up procedu res and made it possible to determine all metabolites without interfer ence on a photodiode array detector. Urine samples collected from Wist ar male rats were analyzed after high-dose oral or intravenous adminis tration of nicotinic acid or nicotinamide. Metabolic pathways of nicot inic acid in male Wistar rats are also discussed. (C) 1998 Elsevier Sc ience B.V. All rights reserved.