CLONING OF CDNA FOR A CELL WALL-BOUND ACID INVERTASE FROM TOMATO (LYCOPERSICON-ESCULENTUM) AND EXPRESSION OF SOLUBLE AND CELL WALL-BOUND INVERTASES IN PLANTS AND WOUNDED LEAVES OF LYCOPERSICON-ESCULENTUM AND LYCOPERSICON-PERUVIANUM

Citation
A. Ohyama et al., CLONING OF CDNA FOR A CELL WALL-BOUND ACID INVERTASE FROM TOMATO (LYCOPERSICON-ESCULENTUM) AND EXPRESSION OF SOLUBLE AND CELL WALL-BOUND INVERTASES IN PLANTS AND WOUNDED LEAVES OF LYCOPERSICON-ESCULENTUM AND LYCOPERSICON-PERUVIANUM, Genes & genetic systems, 73(3), 1998, pp. 149-157
Citations number
57
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Genetics & Heredity",Biology
Journal title
ISSN journal
1341-7568
Volume
73
Issue
3
Year of publication
1998
Pages
149 - 157
Database
ISI
SICI code
1341-7568(1998)73:3<149:COCFAC>2.0.ZU;2-W
Abstract
A cDNA, Wiv-1, for an isozyme of acid invertase (EC 3.2.1.26) was clon ed from wounded leaves of tomato (Lycopersicon esculentum). The encode d protein had a basic isoelectric point and strong similarity to the a mino acid sequences of plant cell wall-bound invertases. The conserved sequence WECPD that is found in all plant cell wall-bound invertases was also found in the deduced protein. These results suggested that Wi v-1 encoded a cell wall-bound acid invertase of tomato. Wounding incre ased the levels of mRNAs for soluble and cell. wall-bound invertases a nd the activities of these invertases in leaves of L. esculentum and o f a related species, L. peruvianum. The induction of Aiv-1 mRNA for th e soluble enzyme in wounded leaves was not very strong, while that of Wiv-1 mRNA for the wall-bound enzyme was prominent. The level of Aiv-1 mRNA reached a maximum 48 h after wounding while that of Wiv-1 mRNA c ontinued to rise for up to 96 h. These findings suggested that the gen es for the two isozymes responded independently to wounding. The level s in various organs of Aiv-1 and Wiv-1 mRNAs were higher in L. esculen tum than in L. peruvianum. Possible roles of cell wall-bound acid inve rtase in wound response and in developing plant are discussed.