CLONING OF CDNA FOR A CELL WALL-BOUND ACID INVERTASE FROM TOMATO (LYCOPERSICON-ESCULENTUM) AND EXPRESSION OF SOLUBLE AND CELL WALL-BOUND INVERTASES IN PLANTS AND WOUNDED LEAVES OF LYCOPERSICON-ESCULENTUM AND LYCOPERSICON-PERUVIANUM
A. Ohyama et al., CLONING OF CDNA FOR A CELL WALL-BOUND ACID INVERTASE FROM TOMATO (LYCOPERSICON-ESCULENTUM) AND EXPRESSION OF SOLUBLE AND CELL WALL-BOUND INVERTASES IN PLANTS AND WOUNDED LEAVES OF LYCOPERSICON-ESCULENTUM AND LYCOPERSICON-PERUVIANUM, Genes & genetic systems, 73(3), 1998, pp. 149-157
A cDNA, Wiv-1, for an isozyme of acid invertase (EC 3.2.1.26) was clon
ed from wounded leaves of tomato (Lycopersicon esculentum). The encode
d protein had a basic isoelectric point and strong similarity to the a
mino acid sequences of plant cell wall-bound invertases. The conserved
sequence WECPD that is found in all plant cell wall-bound invertases
was also found in the deduced protein. These results suggested that Wi
v-1 encoded a cell wall-bound acid invertase of tomato. Wounding incre
ased the levels of mRNAs for soluble and cell. wall-bound invertases a
nd the activities of these invertases in leaves of L. esculentum and o
f a related species, L. peruvianum. The induction of Aiv-1 mRNA for th
e soluble enzyme in wounded leaves was not very strong, while that of
Wiv-1 mRNA for the wall-bound enzyme was prominent. The level of Aiv-1
mRNA reached a maximum 48 h after wounding while that of Wiv-1 mRNA c
ontinued to rise for up to 96 h. These findings suggested that the gen
es for the two isozymes responded independently to wounding. The level
s in various organs of Aiv-1 and Wiv-1 mRNAs were higher in L. esculen
tum than in L. peruvianum. Possible roles of cell wall-bound acid inve
rtase in wound response and in developing plant are discussed.