SINGLE-MOLECULE DETECTION BY LASER 2-PHOTON-EXCITED FLUORESCENCE IN ACAPILLARY FLOWING CELL

Citation
Jm. Song et al., SINGLE-MOLECULE DETECTION BY LASER 2-PHOTON-EXCITED FLUORESCENCE IN ACAPILLARY FLOWING CELL, Analytical sciences, 14(5), 1998, pp. 913-916
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry Analytical
Journal title
ISSN journal
0910-6340
Volume
14
Issue
5
Year of publication
1998
Pages
913 - 916
Database
ISI
SICI code
0910-6340(1998)14:5<913:SDBL2F>2.0.ZU;2-O
Abstract
The two-photon excited fluorescence of fluorescein and Rhodamine B in a flowing sample cell was observed using a self mode-locked Ti-sapphir e laser (180 fs pulse). The detection limit of fluorescein was less th an 2 molecules in the probe volume. The single molecule detection of R hodamine was successfully performed in a flowing cell, and the number of photon bursts agreed approximately with that expected.