EVIDENCE FOR THE ROLE OF LEUCOSEQUESTRATION IN INJURY MEDIATED BY OXYGEN-FREE RADICALS DURING CARDIOPULMONARY BYPASS IN HUMANS

ALATAS J COLAK J DERNEK S SEVIN B INAL M

J. Alatas et al., EVIDENCE FOR THE ROLE OF LEUCOSEQUESTRATION IN INJURY MEDIATED BY OXYGEN-FREE RADICALS DURING CARDIOPULMONARY BYPASS IN HUMANS, Medical science research, 26(9), 1998, pp. 635-637

16

INGLESE

Article

Medicine, Research & Experimental

Medical science research → ACNP

0269-8951

26

9

1998

635 - 637

ISI

0269-8951(1998)26:9<635:EFTROL>2.0.ZU;2-Q

The purpose of this study was to determine the role of pulmonary leuco sequestration in oxygen free radical generation and lipid peroxidation . it comprised 10 patients undergoing cardiopulmonary bypass. Plasma m alondialdehyde levels, erythrocyte superoxide dismutase activity and g lutathione levels were measured in blood samples obtained from the lef t atrium at multiple time points before and during cardiopulmonary byp ass. Erythrocyte superoxide dismutase activity declined throughout the period of measurement. The significant decreases occurred in the isch aemia and early reperfusion periods. Activity rose to control levels i n the late reperfusion specimens. The levels of malondialdehyde and gl utathione and leucocyte count increased significantly, especially in i schaemia and early reperfusion periods (P < 0.001 for malondialdehyde, P < 0.05 for glutathione). These results confirm pulmonary leucoseque stration and oxidative stress during cardiopulmonary bypass. They indi cate that oxygen free radical generation exceeds the capacity of the e ndogenous antioxidant enzyme systems during cardiopulmonary bypass. Me d Sci Res 26:635-637 (C) 1998 Lippincott Williams & Wilkins.