THE P2 PROTEIN OF RICE DWARF PHYTOREOVIRUS IS REQUIRED FOR ADSORPTIONOF THE VIRUS TO CELLS OF THE INSECT VECTOR

Citation
T. Omura et al., THE P2 PROTEIN OF RICE DWARF PHYTOREOVIRUS IS REQUIRED FOR ADSORPTIONOF THE VIRUS TO CELLS OF THE INSECT VECTOR, Journal of virology (Print), 72(11), 1998, pp. 9370-9373
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Virology
Journal title
ISSN journal
0022-538X
Volume
72
Issue
11
Year of publication
1998
Pages
9370 - 9373
Database
ISI
SICI code
0022-538X(1998)72:11<9370:TPPORD>2.0.ZU;2-I
Abstract
Intact particles of rice dwarf phytoreovirus adsorbed to and entered m onolayer-cultured cells of the insect vector Nephotettix cincticeps an d multiplied within the cells. Particles that lacked the PZ protein ne ither attached to nor infected such cells. Furthermore, P2-free partic les obtained from a transmission-competent isolate of the virus were u nable to infect insect vectors that had been allowed to feed on these virus particles through a membrane. However, when such virus particles were injected into insects via a glass capillary tube they successful ly infected the insects, which became able to transmit the virus, Thes e results support the hypothesis that, while P2-free particles can nei ther interact with nor infect cells in the intestinal tract of the ins ect vector, they do retain the ability to infect such cells when physi cally introduced into the hemolymph by injection.