G. Lamche et al., PRIMARY CULTURE OF DISPERSED SKIN EPIDERMAL-CELLS OF RAINBOW-TROUT ONCORHYNCHUS-MYKISS WALBAUM, Cellular and molecular life sciences, 54(9), 1998, pp. 1042-1051
This is the first report on a primary culture of dispersed skin epider
mal cells of rainbow trout Oncorhynchus mykiss Walbaum. These primary
cells revealed a low seeding efficiency after 3 days (11.6 +/- 4.6%),
whereas subcultured cells had a higher seeding efficiency at the same
time point (75.5 +/- 34.0%) and increased in cell number (150-200% of
seeded cells after 20 to 30 days). The cells were characterized applyi
ng histological, immunocytochemical and ultrastructural methods. The c
ulture consisted of undifferentiated keratinocytes. Mucous cells as we
ll as differentiated epithelial cells were absent. To date the cells w
ere cultured for maximally 9 passages and 402 days and therefore provi
de the possibility for long-term studies.