PRIMARY CULTURE OF DISPERSED SKIN EPIDERMAL-CELLS OF RAINBOW-TROUT ONCORHYNCHUS-MYKISS WALBAUM

Citation
G. Lamche et al., PRIMARY CULTURE OF DISPERSED SKIN EPIDERMAL-CELLS OF RAINBOW-TROUT ONCORHYNCHUS-MYKISS WALBAUM, Cellular and molecular life sciences, 54(9), 1998, pp. 1042-1051
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,"Cell Biology",Biology
ISSN journal
1420-682X
Volume
54
Issue
9
Year of publication
1998
Pages
1042 - 1051
Database
ISI
SICI code
1420-682X(1998)54:9<1042:PCODSE>2.0.ZU;2-O
Abstract
This is the first report on a primary culture of dispersed skin epider mal cells of rainbow trout Oncorhynchus mykiss Walbaum. These primary cells revealed a low seeding efficiency after 3 days (11.6 +/- 4.6%), whereas subcultured cells had a higher seeding efficiency at the same time point (75.5 +/- 34.0%) and increased in cell number (150-200% of seeded cells after 20 to 30 days). The cells were characterized applyi ng histological, immunocytochemical and ultrastructural methods. The c ulture consisted of undifferentiated keratinocytes. Mucous cells as we ll as differentiated epithelial cells were absent. To date the cells w ere cultured for maximally 9 passages and 402 days and therefore provi de the possibility for long-term studies.