Analysis of ephedrine in ephedra callus by acetonitrile modified capillaryzone electrophoresis

Citation
Gb. Li et al., Analysis of ephedrine in ephedra callus by acetonitrile modified capillaryzone electrophoresis, TALANTA, 48(5), 1999, pp. 1023-1029
Citations number
10
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
TALANTA
ISSN journal
0039-9140 → ACNP
Volume
48
Issue
5
Year of publication
1999
Pages
1023 - 1029
Database
ISI
SICI code
0039-9140(199905)48:5<1023:AOEIEC>2.0.ZU;2-H
Abstract
A simple method has been developed for the quantitative determination of ep hedrine in ephedra callus. The dependence of effective mobility of ephedrin e on pH was investigated, and a simulated equation was obtained. The separa tion was performed in an uncoated capillary and detected at 185 nm. A new T ris-NaOH-H3PO4 run buffer was used and the pH was adjusted to 3.20. To incr ease the solubility of hydrophobic analytes and improve the separation effi ciency, 15% acetonitrile was used in the buffer as a modifier. The content of ephedrine in an ephedra callus sample and an ephedra herba sample were d etermined with this method, and the result was satisfactory. (C) 1999 Elsev ier Science B.V. All rights reserved.