Background: In the current study the antiinflammatory influence of antithro
mbin III (AT III) therapy on necrotizing pancreatitis was investigated.
Methods: In male Wistar rats (250 g) necrotizing pancreatitis was induced b
y intraductal injection of glucodesoxycholic acid and intravenous infusion
In two experimental groups therapy with 125 IU per kg bodyweigth of AT III
was compared with placebo (Ringer's solution). Survival and pancreatic micr
ocirculation as quantified by intravital microscopy were measured.
Results: 60 minutes after the beginning of AT III therapy in pancreatic cap
illaries there was found a significant higher erythrocyte velocity and func
tional capillary density. (p <0.01). Leukocyte-endothelium-interaction was
also improved significantly in the AT III-group. Survival was not significa
ntly different but there was a tendency for longer survival with AT III the
Conclusion: This study was able to show that the therapy with AT III can im
prove the microcirculatory disorder in necrotizing pancreatitis.
Based on these data a therapeutic increase of plasma AT III-levels may infl
uence the outcome of necrotizing pancreatitis.