Application of molecular methods for detection and transmission analysis of Mycobacterium tuberculosis drug resistance in patients attending a reference hospital in Italy

Citation
A. Cingolani et al., Application of molecular methods for detection and transmission analysis of Mycobacterium tuberculosis drug resistance in patients attending a reference hospital in Italy, J INFEC DIS, 179(4), 1999, pp. 1025-1029
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Immunology
Journal title
JOURNAL OF INFECTIOUS DISEASES
ISSN journal
0022-1899 → ACNP
Volume
179
Issue
4
Year of publication
1999
Pages
1025 - 1029
Database
ISI
SICI code
0022-1899(199904)179:4<1025:AOMMFD>2.0.ZU;2-D
Abstract
A molecular analysis of drug-resistant isolates of Mycobacterium tuberculos is was done in a population with a high prevalence of human immunodeficienc y virus infection. Seventy-one consecutive isolates were tested for genotyp ic resistance to isoniazid, rifampicin, streptomycin, and ethambutol by pol ymerase chain reaction-single strand conformation polymorphism analysis and automated sequencing of target regions. Phenotypic and genotypic resistanc e to isoniazid, rifampicin, streptomycin, and ethambutol were detected in 2 3.4%, 11.2%, 7%, and 5.6% of isolates and in 87%, 88%, 40%, and 100% of res istant isolates, respectively. Specificity was 100% for all target regions. When rpoB, katG, and ahpC mutation analysis were combined, 86% of resistan t isolates to any drug were identified. No mutations in inhA were found in isoniazid-resistant isolates. Molecular detection of drug resistance, parti cularly for isoniazid and rifampicin, may represent a sensitive and very sp ecific technique. The strategy of selecting rpoB, katG, and ahp C to quickl y identify most resistant isolates, with a relevant saving of resources, is warranted.