Separation and direct detection of raw and gelatinized starch hydrolyzing activities of glucoamylase on isoelectric focusing gels

Citation
C. Suresh et al., Separation and direct detection of raw and gelatinized starch hydrolyzing activities of glucoamylase on isoelectric focusing gels, ELECTROPHOR, 20(3), 1999, pp. 483-485
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
ELECTROPHORESIS
ISSN journal
0173-0835 → ACNP
Volume
20
Issue
3
Year of publication
1999
Pages
483 - 485
Database
ISI
SICI code
0173-0835(199903)20:3<483:SADDOR>2.0.ZU;2-T
Abstract
A procedure to detect raw and gelatinized starch activities of glucoamylase on isoelectric focusing (IEF) gels by using 2, 3, 5-triphenyltetrazolium c hloride is described. The reagent reacts with the reducing group of glucose released by glucoamylase from the substrate starch. Using the reaction, pr oduction of glucoamylase by Aspergillus niger was detected on 10% IEF gels within a pH range of 2.5-9.5. Since the method can detect raw and gelatiniz ed starch activities of glucomylase associated with 1 mu g protein, it will be useful for enzyme engineering studies that involve screening of various mutations.